Reactivity of a detergent, polyoxyethylene derivative, with high density lipoprotein subclass

Epidemiological studies have shown that HDL-C is negatively correlated with coronary arteriosclerosis . This is because HDL transports cholesterol from peripheral tissues to the liver, playing a major role in the system for reverse transport of cholesterol (RCT), and having an anti-arteriosclerosis action. The particle size, lipid content, and apolipoprotein components of HDL is heterogeneous. HDL is separated into large HDL2 molecules that have low density and small HDL3 molecules with high density . However, fractions of pre-β-HDL (lipid poor apo A-I) 6) and apolipoprotein E (apo E) rich HDL have also been reported, and it is not clear which fractions they correspond to. There is also much debate about the clinical significance of measuring HDL subfractions. Density gradient ultracentrifugation is the main method of isolating HDL2 and HDL3. In addition Journal of Analytical Bio-Science Vol. 32, No 5 (2009)